| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2009 |
Evaluation of Antiepileptic and Learning Memory activity of Unmadgajakesari Rasa (I) treated in Herbal Drugs |
Dr.Subodh Kele |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
The present study was planned to evaluate acute toxicity, anti-psychotic activity and extra pyramidal side effects of Unmadgajkesari rasa I (UGK-I). The study drug UGK –I consisting of detoxified Mercury, Sulfur, Realgar and Dhattura seeds along with Vacha, Agasti and Bramhi was prepared following SOP as per AFI. Acute toxicity study was performed using Limit Test of the OECD guideline 423. The results revealed that UGK I was nontoxic at 2000mg/kg dose.
In the antipsychotic activity assessed by ‘5-HTP induced head twitches model’ on Swiss albino mice, UGK I when compared to control group showed significant activity however when compared to standard drug [olanzapine] did not show significant activity. UGK-I was also assessed experimentally to check if it produces extra pyramidal side effects like catalepsy where it proved not to produce catalepsy. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2012 |
Evaluation of Nootropic Activity of Hingvadi Ghrita In Experimental Animal Models. |
Dr. Priyanka Padavi |
Dr. Nandini More, Associate professor BVDUCOA |
NA |
Hingwadi Ghrita (HG) which is used to treat unmad and apasmara contains Hingu, Sauvarchal lavan, Trikatu, Gomutra and Goghrita. Cumulative effects of this formulation is seen as Medhya, Smritikar and Agnivardhak. Also, the individual ingredients of the present formulation have been reported to possess antioxidant, anti-depressant & memory enhancer activities. In this study HG was evaluated for its nootropic activity after taking permission from institutional animal ethics committee; using Elevated Plus Maze(EMP) in mice & Morris Water Maze (MWM) in rat. The three doses used in EPM model were 2.5g/kg, 5.0g/kg, 10g/kg respectively. For MWM 1.8g/kg, 3.6g/kg, 7.2g/kg respectively were the dose levels. For both the tests standard comparator drug piracetam at dose 7.8g/kg in mice and 270g/kg for rats was used whereas Goghrita 5.0 g/kg in mice and 3.6g/kg in rats was used as vehicle control. The plain control was treated with distilled water. The test drug was administered orally to all animals in each group for the duration of 21 days. Diazepam was used in 1mg/kg dose by i.p. to induce amnesia on day only for EPM model. Assessment criterion for EPM was decrease in transfer latency (TL). For MWM there were two criteria, decrease in escape transfer latency (ETL)and number of annulus crossing. The data was analysed using paired T test and one way Annova followed by Dunnett's test. HG at 5.0g/kg dose showed statistically significant (p<0.001) results for EPM model. HG at 3.6gm/kg dose showed significant (p<0.001) decrease in ETL. Additionally it is noted that HG is better than cow ghee but equi-effective with piracetam. It can be concluded that HG shows nootropic potential. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2006 |
Experimental Toxicological Evaluation of Aadrak Swaras Bhavita Shuddha Manahshila |
Dr. Sandeep Ubale |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Ashuddha (Raw) Manashila shows untoward effects and Shuddha Manashila is used as a safe medicine. Ardrak Swaras Bhavana is responsible for this conversion. Shuddha Manashila was prepared using 7 Bhavanas of Ardrak Swaras. Toxicity profiles of Ashuddha and Shuddha Manashila were established and compared to evaluate role of Ardrak Swaras Bhavana using OECD guidelines 423 and 407 for Acute and Sub acute toxicity respectively. For the same purpose Samples of Shuddha and Ashuddha Manashila were compared. Acute Toxicity study revealed that Ashuddha Manashila is non toxic at 5000mg/kg dose. With this data instead of acute toxicity study, sub-acute toxicity study of Ashuddha and Shuddha Manashila was conducted. Wister Rats were fed for 28 days considering their body weights. On 29th day Blood samples were collected, animals were sacrificed by cervical dislocation and dissected organs were sent for histo-pathological investigations.Thus, out of 8 parameters studied significant abnormalities were not observed in 7 parameters viz. average body weight per group, general observations, food consumption, hematological investigation, percentage organ weight, gross necropsy and histo-pathological investigations and in case of parameter Biochemical investigations out of 18 values studied 14 values were found to be normal in all animals from all groups. Remaining 4 biochemical values viz. Urea, |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2009 |
Toxicity study of Unmadgajakesari Rasa [UGK I] and its Antipsychotic activity in Animal Models |
Dr.Prashnat Nimbalkar |
Dr. Madhuri Dalvi-Pawar, associate professor |
NA |
Unmadgajakesari rasa(UGK I) is one of the herbo-mineral formulations specially recommended in the treatment of Unmad [Psycosis] and Apasmar [Epilepsy]. It contains three mineral drugs i.e detoxified Mercury, detoxified Sulphur and detoxified Manashilla, and four herbal drugs i.e. detoxified Dhatura (Datura semina) seeds, ‘Vacha kanda’ Kwath, ‘Agasti patra’ Swaras and ‘Brahmi whole plant’ Swaras. A unique manufacturing process i.e “Bhavna” (wet trituration) is conducted to formulate a standard drug. It helps to reduce the particle size and dose of the final formulation. Combination of herbo-minerals is a ‘synergism’ plays an important role to increase target action and therapeutic efficacy of formulation.In the study attempt was made to manufacture standard drug andassess its Antiepileptic and Learning memory activity (LM). For anti epileptic activity two animal models viz Electro Shock and PTZ induced convulsion model in mice were used. In Electro shock model UGK I was administered in two drug dose levels i.e. X=1.95 mg, 2X=3.9 mg. It showed that test drug was equi effective as compared with standard (Phynotoin) drug. In PTZ model at same drug dose levels UGK I showed statistically significant effect compared to control group. UGK I was not significant as standard drug. Cook’s and widlies model was used to access learning and memory activity in rats. LM activity was analyzed by using Anova test it is resulted that test drug UGK I is highly significant in LM activity. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2011 |
Experimental Evaluation of Antipsychotic Activity of Brahmyadi Ghrita. |
Dr. Nashir Parmar |
Dr. Nandini More, Associate professor BVDUCOA |
Dr. Beri, Assistant professor, BVDU, Medical College |
Schizophrenia is a psychosis characterised by delusions, hallucinations, and lack of insight, it may present with disturbed behaviour, marked delusions, hallucinations, and disordered thinking or with social withdrawal. Antipsychotic drugs are the main stay of treatment of schizophrenia but are also associated with various side effects like dry mouth, diarrhoea, constipation, tardive dyskinesia, memory loss, uncontrollable body movements, muscle weakness, suicidal thoughts, and weight gain. Brahmyadi Ghrita (BG) is a polyherbal formulation and recommended to treat various CNS conditions in Ayurved. In this study, BG was prepared and analysed physico-chemically and was evaluated for its antipsychotic activity using 5-HTP induced head twitches in mice at dose levels (2.5, 5, 10) gm/kg. It was also evaluated for its extra pyramidal adverse effect using catalepsy in mice model. Data was analysed by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple group comparison test. BG at all three dose levels showed better antipsychotic activity by reduction in number of head twitches in 5HTP model when compared to control group (p≤0.001). Catalepsy which is a known adverse effect of antipsychotic drugs was not observed in all three test groups. In conclusion, Brahmyadi ghrita has antipsychotic action without any extra pyramidal effect. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2015 |
Comparative assessment of antidepressant activity of Brahmi Ghrita prepared from Puran Ghrita and Fresh Goghrita using CUMS. |
Dr. Bhavana Menkudale |
Dr. Madhuri Pawar, Professor , BVDUCOA |
Dr. Jayashree Dawane, Associate |
The present study compared Brahmi Ghrita prepared from Puran ghrita and fresh go-ghrita on physicochemical parameters. Materials and methods: In accordance to ayurvedic standard protocols, two samples of Brahmi ghrita were prepared with Puran Ghrita (BG I) and fresh cow ghee (BG II). Further validation and comparative physico-chemical analysis was done for both samples. The physico-chemical parameters such as specific gravity, moisture content, acid value, iodine Results: value, saponification value and peroxide value of BG I and BG II samples were almost alike. The process Conclusion: of manufacture and analytical values of BG I and BG II samples are validated and established in the study. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2005 |
Comparative Experimental Evaluation of Talisadi churna and Guti Containing Natural Vanshalochan |
Dr. Alam Shahawar |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
In the present study Identity of Natural Vanshlochan was established by performing Physico-chemical tests, XRD, TGA and SEM-EDAX techniques. Standardized Talisadi churna and Guti containing Natural Vanshlochan were prepared. These two dosage forms were studied in Bronchial Asthma.
For a formulation to be effective in Asthma, it has to possess both anti-histaminic and anti-inflammatory activity. To study Anti-Histaminic activity, animal model ‘Protection against histamine induced bronchospasm’ was selected, in which 155 mg/kg/day dose was of TC and TG was extrapolated for guinea pigs. To study Anti-Inflammatory activity animal model ‘Cotton wool Granuloma’ was selected wherein 180 mg/kg/day dose of Tc/TG was extrapolated for Rats. It was concluded that Talisadi churna and Guti both have significant Anti-Histaminic and Anti-Inflammatory activity. When Talisadi churna and Guti were equally effective for both the activities. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2016 |
Evaluation of three dosage forms of Guduchi on learning and memory in young and aged Mice |
Dr.Vaidehi Patankar |
Dr. Madhuri Pawar, Professor , BVDUCOA |
Dr. Swati Gadgil, Assistant Professor, BVDUCOA,Dr. Dawane Jayashree,Associate Professor, BVDU Medical College |
In Ayurved, Guduchi (Tinospora cordifolia) is advocated as Medhya Rasayan (Cognitive enhancer). It is emphasized to use fresh form of swaras of Guduchi (Extracted juice) to attain memory enhancing effect on human cognition. However Guduchi swaras(GS), has less shelf life and extreme bitter taste hence to overcome this lacunae Guduchi Kalpa (GK) which sugar coated granule form and Guduchi ghrita (GG) i.e. medicated ghee from was prepared. By applying Ayurvedic standard protocols GS, GK and GG was formulated and Method of preparation were developed and validated through physicochemical analysis. To assess the effect of these formulations Diazepam induced amnesia in Elevated plus maze (EPM) and spatial learning in Radial arm maze (RAM) animal models were used . For the assessment of anti amnesic activity , test drugs at the dose of GS 20ml/70kg, GK 40gm/70 kg and GG 40gm/70kg were administered orally for 14 consecutive days. In EPM test, antiamnesic activity of three formulations of Guduchi is confirmed as decrease in transfer latency (TL) of animals and Significant reversal of amnesia was noted in GK when compared against Induction control group. While in RAM test, GS and GK exhibited significant action on increase percentage of correct choices and reduction in working and reference memory errors in comparison to GG at same dose level . Hence the present study has created an evidence of anti amnesic and nootropic activity of GS and GK in Diazepam induced amnesia and Spatial learning in radial arm maze animal models. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2013 |
Clinical Assessment of Brahmyadi Ghrita for Improvement in Learning and Memory of High School Children. |
Dr. Prachi Patki |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Medha is a combination of cognitive processes of brain, of which learning and memory are
important factors. Study was aimed to evaluate the efficacy of Vachadi Ghrita as memory
enhancer. The objective of the study was to manufacture Vachadi Ghrita and to assess its
efficacy in healthy high school children for whom two validated learning and memory tests were
used. ‘Numerical Ability Test’ measures lower cognitive skills and ‘Higher Mental Ability in
Science’ measures higher cognitive skills. Vachadi Ghrita was prepared following SOP and
analytical values of VG were matched with the established standard values. Tests were taken
before & after completion of ghrita consumption. Selected healthy students were non-randomly
divided into 3 groups of 30 students each Group A - Study Group-Vachadi Ghrita, Group B -
Positive Control-Goghrita, and Group C - Plain Control-no drug. Group A and Group B students
were received respective Ghrita in a dose of 10 gm with powdered sugar in morning on empty
stomach followed by lukewarm water for 30 days continuously. Primary outcome of study is
‘increase’ in tests score. Scores were statistically analysed using paired‘t’test for intra-group
comparison and ANOVA followed by tukey’s for inter group comparison. Secondary outcome of
study was ‘changes in certain secondary subjective and objective parameters’, which were
assessed on the basis of ‘Samyak Snigdha Lakshana’. In present study VG at a dose of 10gm/day
for 1 month had shown highly significant improvement in simple cognitive skills as compared
with Goghrita at p <0.05. Increase in appetite (26%for VG and 28%Goghrita), easy evacuation
of bowel (75%), no change in weight (p<0.05) were seen in both the groups. Palatability was
found to be reduced due to aversion and nausea in VG group. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2014 |
Clinical Evaluation of Brahmyadi Ghrita in Mild to Moderate Depression. |
Dr. Nishigandha Chaudhari |
Dr. Nandini More, Associate professor BVDUCOA |
NA |
Brahmyadi Ghrita (BG) from Ashtanghridayasamhita comprising of Brahmi, Siddharthak, Vacha, Sariva. Kushtha, Pippali and Saindhav lavan processed in cow ghee is used to treat various CNS conditions. The present study was undertaken to standardized BG and investigate its antidepressant effects. The BG complied with API parameters having pH 7, sM.D. 0.945, saponification 293.1, acid value 2.46, peroxide value 1.7, free fatty acids 0.78, and total fats 94.65. The patients of mild to moderate depression of age group between 25-50 years were included in the study after informed written consent. The patients were randomised into study and standard control groups using lottery method. BG was administered in the dose of 10gm twice a day with lukewarm water for 60 days. Hamilton’s Depression Rating Scale test scores were recorded on days 1, 30, 61 and were analyzed statistically by using Friedman test for within group and Mann Whitney test for between group comparison. The antidepressant effect of Brahmyadi Ghrita was significantly better (p<0.005) than the conventional antidepressants, after treatment of 60 consecutive days. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2004 |
Effect of concentration and pharmaceutical method on pharmacological activity of Shwaskuthar rasa with respect to Marich |
Dr. Avinash Chaugule |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Background and aim: In the present work, activity against histamine induced bronchoconstriction of four samples of standardized Shawskuthar Rasa [SK] was studied.
Methodology: The study was conducted on guinea pigs [GP] using the model ‘protection against Histamine induced bronchospasm’ as the species is very sensitive to histamine.
Total 30 guinea pigs were classified into 5 groups, each consisting of 3 males and 3 females, for samples S1, S2, S3, S4 and control. The drugs viz. S1, S2, S4 in the dose 29.05mg/kg & S3 in the dose 13.95mg/kg mixed with honey (Anupan), and only a drop of honey to control group; were administered orally using spatula with utmost care to avoid any wastage, for 21 days to each animal in the respective group. The guinea pigs were exposed to histamine aerosol challenge using 2% histamine solution through a nebulizer attached to the histamine chamber on 22nd day. Time required to produce Pre convulsive dyspnea [PCD] and the quantity of Histamine solution required to produce mist was noted for each GP. The animals were revived using adrenalin. The results were compared between the groups using ANOVA.
Results: The average time required to produce PCD was 6.32 minutes. 5.50 minutes and 4.21min in S1, S2, S3 samples as compared to the 2.54 min in control group. Time required to produce PCD in male GP was 3min and that of in female GP was 10 min in group S4. This group was not included in statistical analysis as it needs further probing.
Conclusion: P value of 0.0000001 highlights that SK has antihistaminic activity and the effect varies according to the proportions of ingredients and method of manufacture. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2014 |
Evaluation of antidepressant potential of Panchagavya Ghrita as an adjuvant in patients of Depression. |
Dr. Viraj Bhandari |
Dr. Swati Gadgil, Assistant Professor, BVDUCOA |
NA |
Depression is a widwspread psychiatric disorder affecting around 5%of the population and can be estimated to be second largest global burden of disease after hear disease by 2020.Although currently prescribed molecules for depression improve the clinical condition of patient but, it is at the worth of bearing of its adverse effect like sleep disruption,sexual dysfunctionand weight gain. Panchagavya ghrita is one of the frequently and consistently used formulations in Ayurvedic fraternity to treat various neuropsychological disordes. Panchgavya ghrita was preapred following SOP and analytical values of M.D.G were match with the established standard values. Selected prediagnosed patients of depression were non randomly divided into 2 groups of 6 patients in each group. Group A- Study group - conventional therapy along with the Panchgavya ghrita, Group B- Conventional therapy.Decreases in the score of by atleast 1 unit hamilton scale test was used as the primary outcome measure. treatment was given for duration 60 days. Every patient was assessed pre, med, post treatment using friedmen tesr.Between groups comparison was done using Mann whitney test. Secondary outcome measure for study by changes in certain subjective parameters. Panchgavya ghrita proved as an adjuvant drug by showing antidepressant potential in the patients of depression when compared with only convential therapy |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2016 |
Comparative assessment of nootropic activity of Yashtimadhu churna and Kshirapaka in Young and Aged Mice |
Dr. Priyanka Mohite |
Dr. Madhuri Pawar, Professor , BVDUCOA |
NA |
Yashtimadhu is one of the Medhya Rasayan drugs advocated to achieve desired effect on cognitive functions especially when given with Cow milk. In the present study two dosage forms of Yashtimadhu i.e. churna [Powder] and Kshirapaka [Medicated Milk] were prepared as per standard guidelines given in ayurvedic classical text and were analysed with API parameters. Therefore, it can be said that a preparation method of both formulations is developed and validated with physic-chemical analysis. In extension, comparative assessment of Nootropic activity of both formulations was done by using two animal models, viz. Diazepam induced amnesia using elevated plus maze [EPM] in young mice and spatial learning and memory in radial arm maze [RAM] in aged mice. In EPM test and RAM test drug administration was done daily for 15 and 7 days respectively. Study findings suggest that Yashtimadhu Kshirpaka at therapeutic dose exhibited anti-amnesic effect and learning-memory enhancement action in EPM test and RAM test (P<0. 001). The result also specified comparative significant effect of Yashtimadhu churna with cow milk on animal’s cognition than Yashtimadhu churna with water. Thus it can be concluded that addition of cow milk as Anupana [Vehicle] to Yashtimadhu or processing of Yashtimadhu churna with cow milk in Kshirapaka formulation has potential to maintain healthy cognitive process in animals’. Hence both potent formulations can be employed as nootropic drugs to maintain human cognition in balanced state or can be utilized as adjuvant to conventional treatment. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2008 |
Standardization of Vasarishta containing Mritasanjeevani Sura |
Dr. Nikhil pendase |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Vasarishta built upon Mritasanjeevani sura is a polyherbal hydro alcoholic formulation [Sandhana kalpa] prepared by fermentation process. It is noteworthy that this Vasarishta is administered in a dose of 1 ml as against standard dose 40 ml for any other sandhan kalpa. The present study was aimed at finding out the difference reflected in Vasarishta samples manufactured by standard process employed by pharmacies and the peculiar process documented in Bhaishajyaratnawali. It is also attempted to discuss about the rationale for the process. All the raw drugs complied with the Ayurvedic Pharmacopeia of India (API) standards. The peculiar distillatory, which is seldom used in making of asavarishta, was assembled for preparation of Mritasanjeevani sura (MS). Three batches of MS and subsequently 3 batches of Vasarishta were prepared following SOP. Organoleptic, physicochemical tests like pH, percentage of Alcohol and TRS, specific gravity were conducted aseptically on both MS and Vasarishta , during and after completion of process. Thus basic standardization and development of SOP of Vasarishta built upon Mritasanjeevani sura (MS) was established. Lastly, MS and Vasarishta (built upon MS) were compared with market samples of MS and Vasarishta prepared as per standard method to find out differences using Gas Chromatography.
The types of alcohols and volatile acids in MS and Vasarishta, prepared in lab, are similar but the amounts differ. This is the indicator of process standardization. Values of furfural, ethyle acetate and 1- butanol in lab samples are within permissible limits as against the values of both the market samples. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2012 |
Experimental Evaluation of Panchagavya Ghrita on Depressive Disorders. |
Dr. Tapas Nikhare |
Dr. Swati Gadgil, Assistant Professor, BVDUCOA |
NA |
Depression is a widespread psychiatric disorder affecting around 5% of the population and can be estimated to be second largest global burden of disease after heart disease by 2020. Panchagavya ghrita (M.D.G) is one of the Ayurvedic “Snehapaka formulation’’ (cha. chi. Cha.15)which contains five ingredients obtained from Cow. M.D.G was proven to possess hepatoprotective, anticonvulsant, Anti-epileptic and Antioxidant activity. In this study it was evaluated for its Antidepressant activity using Forced Swim Test (FST) & Tail Suspension Test (TST). Amoung 6 groups 3 were of M.D.G (1.8mg/kg, 3.6gm/kg, 7.2gm/kg respectively in rats & 2.5gm/kg, 5.0 gm/kg, 10 mg /kg respectively in mice). For both the tests, imipramine (10mg/kg in rats & 2.6mg/kg in mice) was used as a standard control where as Goghrita (3.6gm/kg in rats & 5.0gm/kg in mice) was used as a vehicle control and plain control was treated with distilled water. All the drugs were administered orally for the duration of 21 days. Data was analysed using Paired T-test and One way Annova followed by Dunnett’s test. In FST at 5.0 gm/kg and TST at 3.6gm/kg dose, M.D.G showed statistically significant results comparable as that of imipramine. So it can be concluded that results of the present study indicate the potential for use of M.D.G as an adjuvant in the treatment of depression |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2010 |
Experimental evaluation of Antipsychotic activity of Hingvadi Ghrita prescribed in Unmad. |
Dr. Harshita Nagtode. |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Schizophrenia can be correlated with a disease ‘Unmad’ stated in Ayurved as the signs and symptoms of both diseases are similar. ‘Hingwadi Ghrita’ (HG) is one of the formulation containing four herbal drugs and two animal source drugs advocated in the treatment of Unmad and Apsmar. The present study is aimed at assessment of Antipsychotic activity of fresh prepared standard Hingwadi Ghrita and its extra pyramidal adverse effect if any.
All the ingredients of HG complied with the standard parameters mentioned in Nighantu texts and API. Standard values of HG were established in this study by following standard protocol. For antipsychotic activity two animal models viz. ‘Apomorphine induced climbing in mice’ and ‘5 HTP induced head twitches in mice’ were used. For extra pyramidal adverse effect catalepsy in mice model was used. Test drug HG in three dose levels (X/2) 0.05 gm, (X) 0.1 gm and (2X) 0.2gm for a 20gm mouse was compared with standard drugs Haloperidol and Olanzapine. Goghrita was used as sham control.
Antipsychotic activity study revealed statistically significant results [p<0.001] in both mice models. It does not produce catalepsy which is a known adverse effect of antipsychotic drugs. Thus it can be concluded that antipsychotic activity of HG may be mediated through blocking 5HT receptor and mesolimbic pathway. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2005 |
Pharmaceutical and Experimental Study of Talisadi churna and Guti Containing Synthetic Vanchalochan |
Dr. Atul Gaikwad |
Dr. Nandini More, Associate professor BVDUCOA |
NA |
Vanshlochan formed only in female species and after every twelve years of flowering period,is on the verge of extinction. It’s synthetic substitute is widely used among practitioners and pharmaceutical industries.Synthetic vanshalochan was subjected to physicochemical tests and further the churna and guti prepared from this were studied using ‘Protection against histamine induced bronchospasm’ and ‘Cotton wool Granuloma’ models. Animals were classified into three groups of 6 animals -3 males and 3 females each. One was control group and two groups were study groups. Analysis of synthetic vanshlochan by XRD revealed its amorphous form, images were obtained using scanning electron microscope. EDAX results confirm presence of O, Na, Mg, Al, Si, S, K, Ca, Fe, in synthetic vanshlochan.TGA showed the thermal stability of synthetic vanshlochan at 610 degree. Standarization values of churna and Guti matched to the standards of API and IP respectively. For, antihistaminic activity Mean time required for pre convulsive dyspnoea was greater in Talisadi churna and guti as compared with control. Similarly for anti-inflammatory activity mean weight of granuloma formed was less in both Talisadi churna and guti as compared to control. No significant difference was observed in both the activities of churna and guti. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2009 |
Evaluation of Acute Toxicity and Antipsychotic Activity of Unmadgajakesari Rasa-II: A Herbo-Mineral compounds |
Dr.Jyoti Singh |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
It has been estimated that by the year 2020, nearly 15% of the world’s disease burden will be due to mental disorder1. Widely studied disease, Schizophrenia can be correlated with Unmad2 on the basis of signs and symptoms. Unmadgajakesari rasa 3[UGK II], a potent herbo-mineral formulation which is used clinically yet has no documented evidence of safety and activity has been taken for study here. The study aimed at standardization of pharmaceutical process of UGK II preparation by puta method; evaluation of its toxicity profile, pharmacological activities, and adverse effects.
The Standard operating procedures [SOP] for manufacture of UGK II including pharmacognosy of ingredients, optimisation of ‘puta’ method and confirmatory tests of intermediate products were followed and confirmed. Acute toxicity study of UGK II was conducted using OECD guideline 4235. ‘5HTP induced head twitches’6 model was used to evaluate antipsychotic activity. Extra pyramidal adverse effect was evaluated on the model ‘catalepsy in mice’7.
UGK II complied with the standard parameters as per API and other texts. Acute toxicity study revealed that UGK II was nontoxic at 2000mg/kg dose. It shows statistically significant [p<0.001] antipsychotic activity in 5HTP induced head twitches model. It does not produce catalepsy [p< 0.001] which is a known adverse effect of antipsychotic drug. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2007 |
Efficacy of Devdarvyadi Dhoomavarti in Avegavastha of Tamak Shwas |
Dr. Mukesh Chandra |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Among different therapeutic procedure Dhumpana has got its own importance as the medicine is directly delivered into the pranvaha strotas and thereby initiates rectification in pathology.For the same reason the response to the treatment is likely to be spontaneou. At the same time it also cause srotomardava( lubrication of passage), strotoshodhan further facilitating the clarity of the pranvaha strotad(pranvilomata). Asinhalation (Dhumapana) clears the pranavaha strotas , the free movement of pranvayu is possible resisting the attacks of Tamak Shawas(TS). Patients were categorized in to trial and control groups by Random Lottery method and trial group patients were given 3 Apaan ( inhalation) of Devdarvyadi Dhoomvarti(DDV) with modified Dhoomyantra for 3 times at the interval pf five minutes in the morning for 14 days. Analysis for evalution of the sign, symptom, Spirometric observation and haematological investigation done by students paired t test and population domain is analyzed by applying students unpaired t test to check whether the patients were from same category of criteria. Snaihika Dhoompana chikitsa in chronic TS is the better option in relieving the symptoms as well as prolonging the Recurrence by augmenting the Bala (Strength) of the respiratory tract. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2015 |
Comparative evaluation of antidepressant activity of Brahmi Ghrita and Avartit Brahmi Ghrita using CUMs animal model |
Dr. Vidushi Sharma |
Dr. Madhuri Pawar, Professor , BVDUCOA |
Asmita Wele, Swati Gadgil |
introduction:With a rising need for development of drugs that are effective with multifaceted therapeutics for the treatment of depression and the associated behavioral changes, brāhmī ghr̥ta offers a choice of treatment. The challenge of palatability of ghr̥ta in therapeutic dose of 20 to 40 g per day can be overcome by potentiation and the same was tested in the present study. Aims: To evaluate and compare the efficacy of 1x Brāhmī Ghr̥ta (BG) and 10x BG in Chronic unpredictable mild stress (CUMS) induced behavioral alterations. Settings and Design: Randomized Controlled preclinical trial. Materials and Methods: Self prepared and standardized 1x Brāhmī ghr̥ta (3.6 g/kg) and 10x BG (3.6, 1.8 and 0.9 g/kg) were evaluated in CUMS induced behavioral changes in rats by using sucrose preference (SPT), forced swim (FST). Statistical Analysis Used: One-way ANOVA followed by Post hoc analysis. Results: 28 days CUMS procedure induced depression in rats which was confirmed. Upon comparison with CUMS control group, 1x BG and 10x BG, at all three dose levels, increased the sucrose preference (P < 0.05); in forced swim test increased the number of rotations (P < 0.001. Further, 10x BG at all 3 dose levels showed significantly better results in comparison to 1x BG in FST (P < 0.01) . Conclusions: Both the samples of brāhmī ghr̥ta āēapākī (1x) and daśapākī (10x), showed antidepressant effect. This is the first study to report that drug potentiation increases the efficacy and achieves dose reduction. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2012 |
Experimental Evaluation of Antidepressant Activity of Hingvadi Ghrita Using Animal Models. |
Dr. Poonam Gupta |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
The present study was done to evaluate Anti- depressant activity of Hingwadi Ghrita in vivo. It was a comparative preclinical study. Hingwadi Ghrita (HG) was prepared using standard operating procedure, physicochemically analyzed and assessed. Tail suspension Test (TST) model with Swiss albino mice and Forced Swim Test (FST) model with Wistar albino rats were used to assess anti-depressant activity. Imipramine hydrochloride in dose of 15mg/kg for TST and 10mg/kg for FST, was the standard drug and Ghee as vehicle control in dose of 0.1g/20g for TST and 0.72g/200g for FST orally. Hingwadi Ghrita in doses of 0.05g/20g (x/2), 0.1g/20g (x) and 0.2 g/20g(2x) for TST and 0.36g/200g (x/2), 0.72g/200g (x) and 1.44g/200g (2x) for FST was administered to 3 test groups for 21 days orally except Plain control group which received only distilled water. Duration of immobility in seconds for TST and number of rotations for FST were noted for assessment.
Statistical method used was One way Anova followed by Dunnets test and Paired t test. Results showed that HG was significantly effective at dose of 0.1gm/20gm for TST (p=0.0037; p<0.01) and 0.72g/200g for FST (p=0.0055, p<0.01) comparable to Imipramine hydrochloride. It can be concluded that HG displayed potent anti depressant activity. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2011 |
Experimental Evaluation of Antipsychotic of Vachadi Ghrita using animal Models. |
Dr. Amol V. Sonune. |
Dr. Asmita Wele , Professor, BVDUCOA |
Assistant professor BVDU, Medical College |
The aim of this study was to investigate the Vachadi Ghrita for its antipsychotic activity by using 5HTP induced head twitches in mice model and for its extra pyramidal side effects if any by using catalepsy in mice model. VG is formulated by processing paste of Vacha (Acorus calamus), Guduchi (Tinospora cordifolia), Shati (Hedychium spicatum), Shankhapushpi (Convolvulus pluricaulis), Haritaki (Terminalia chebula), Vidang (Embelia ribes), Shunthi (Zingiber officinale), Apamarg (Achyranthes aspera); in cow ghee and water. SoP and standards were established. The dose used was 0.1 gm/20 gm mice and administered only once in a day. The study was carried out in three dose levels as VG X/2 (0.05 gm/20gm), VG X (0.1 gm/20gm), VG 2X (0.2gm/20gm). the standard comparators wele olanzapine and Haloperidol for 5 HTP induced twitches and Extrapyramidal effect respectively. Data obtained were analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparison test. P<0.001 was considered statistically significant. The moderate antipsychotic action of Vachadi Ghrita is probably mediated through blocking 5HT receptors as well as through Mesolimbic dopamine pathway. Vachadi Ghrita does not show cataleptic action, hence it is not likely to act through Nigrostrital dopamine pathway. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2007 |
Standardization of Pathadyasav used in Shwas [Bronchial asthama] |
Dr. Neelakantha Khadtar |
Dr. Madhuri Dalvi-Pawar, associate professor |
NA |
Pathadyasav is an ayurvedic fermented formulation, prescribed for respiratory disorders. It contains Suramanda prepared from (Rice grains), Guda (Jaggery) and herbal drugs viz Patha Murva, Rasna, Saral, Devdaru. The scientific preparation method and quality parameters of PS are not documented yet. Thus present study is aimed to develop and validate the preparation method and to generate quality standards for Pathadyasav. Preparation of Sura, Suramanda and PA was done as per standard protocols of ayurvedic pharmaceutics blended with modern techniques. All herbal drugs, Sura and PA were tested with standard analytical methods. Obtained values for herbal drugs were within limits as per API standards, signify purity of plant materials. Study result provides organoleptic, physico-chemical values and gas chromatography quality standards for Sura and Pathadyasav. Standard operating procedure for preparation of Sura and Pathadyasav is developed and validated by using standard methods of analysis. It could be used in further studies. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2014 |
Study the therapeutic effect of Vachadi Ghrita in patients of Depression. |
Dr. Rashmi Kulkarni |
Dr. Madhuri Pawar, Associate Professor , BVDUCOA |
NA |
Depressive disorders universally affect wide population due to causative factors such as irregular food habits, peer pressures and mental stress. Traditional Ayurveda literature reveals thatthe pre-monitory symptoms of Unmada [Insanity] and Vishad term used in ayurvedic classics can be corelated with symptoms of depression, denoting restricted activities of speech, body and mind. In management of these disorders Ayurveda explains various medicated ghee formulations which are fore most drug of choice due to its lipophilic nature. Vachadi ghrita (VG) is one of the medicated ghee formulations, contains eight herbal drugs along with Cow ghee. In this study assessment of therapeutic effect of VG was done in mild to moderate patients of depression using Hamilton scale test (HAM-D). Study group patients received VG plus standard antidepressant drugs while patients from control group received standard antidepressants. Significant change in HAM-D scores was noted after 60 days treatment of both groups when compared with their base line scores. However, no statistical significant difference was noted in HAM-D scores of studies, drug group when compared with HAM-D scores of standard drug group. As a result, it can be said that in this exploratory study VG didn’t show positive effect as adjuvant drug in treatment of mild to moderate depression. Moreover, certain encouraging effects came across with administration of study drug which showed increase in patient’s appetite and bowel evacuation easily as compared to standard drug. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2011 |
Experimental Evaluation of Nootropic activity of Brahmyadi Ghrita using animal models |
Dr. Megha Sangwan |
Dr. Swati Gadgil, Assistant Professor, BVDUCOA |
Dr. Kanchan Borole Assistant professor, BVDU, Medical College |
Brahmi is a very well-known plant for its various activities on CNS and is traditionallyused since centuries in Ayurveda. It is used as a single drug or in combination withother herbs. Apart from routinely used formulation BrahmiGhrita, a different onetitledBrahmyadiGhrita comprising of Brahmi, Siddharthak, Vacha, Sariva, Kushtha,Pippali, Saindhavlavan; processed in cow ghee is praised in classical text especiallyfor various CNS conditions of children.Thus, the present study was undertaken to manufacture& standardization of standarddrug(BG) andinvestigatesthe effects on cognitive functions. Nootropic activity wasstudied using Morris Water Maze test in rat and Diazepam induced amnesia in EPMmice model. BG produced a dose dependent improvement in memory scores. BG alsoreversed successfully the amnesia induced by Diazepam (1mg/kg, i.p.).BG wasadministered in three dose levels i.e. X/2 (0.05mg), X (0.1mg) and 2X (0.2mg) per20gm mice. It shows that BG at X dose is equi-effective when compared withPiracetam in EPM model. To assess the effect of BG on learning &memory, MWM in ratmodel was used. BG was administered in three dose levels i.e. X/2 (0.36gm), X(0.72gm) and 2X (1.44) per 200gm rat. In this model it showed that learning andmemory is significantly (p < 0.001)retained in rats after 7 & 14 days of BGtreatmentcompared to standard drug. The nootropic effect of BG was observed in both theexperimental models. Therefore, its worthwhile to explore the effectiveness of BG inCNS conditions.Keywords: Brahmyadighrita, Morris water maze, Nootropic activity,MWM, Elevatedplus maze. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2004 |
Bioavailability and efficacy of Marich (Piper nigrum,Linn) in Shawskuthar Rasa with variation in concentration and manufacturing process |
Dr. Trupti Patil |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Background and aim: To establish data of pharmacokinetics of complex herbomineral formulations; the bioavailability of Shwaskuthar Rasa is explored using mercury as a marker. Also the effect of amount of Marich and method of processing on bioavailability is assessed. A comparative efficacy of 3 samples of Shwaskuthar Rasa varying in proportions of ingredient and one by one trituration technique; is assessed in patients of Tamakshwasa against a widely used market sample. Methodology: Two standard samples of Shwaskuthar Rasa; varying in proportion of Hg 7.14 % and 11.1 % and that of Marich 60% and 22.2 % in S1 and S2 respectively were studied. After administration of 250 mg dose of the S1 and S2 to 8 patients, blood samples were collected at 0, 1, 1.5 and 2 hours and tested by atomic absorption spectroscopy [AAS] coupled with hydride generation technique for mercury content. Further, a single blind randomized controlled clinical trial is conducted at the BV Ayurveda hospital in 24 case of Tamak Shwas. Patients of one yr chronicity and Kapha pradhan cases with age above 16 yrs, were included. Vata pradhan shwasa type and patients on maintenance steroids, Cardiac asthma and other complications were excluded. Formulations S1, S2, and market sample S4 were administered in 125 mg TID dose and S3[prepared using Marich extract] in 60 mg TID dose with warm water as Anupana at Rasayan, Vyan, and Udan Kala ie early morning, before lunch and dinner for 45 days. Follow up was taken every 15 days. Reduction in severity of Tamak shwasa was recorded by score card method before and after treatment. The data was analyzed by employing median test. Results: It is evident that mercury gets absorbed from both the formulations in trace levels. There is no significant difference in absorbance at 1.5 hrs [P= 0.038607] but there is highly significant difference in absorbance at 2 hrs [P= 0.002248]. Hg level from S1 is significantly higher than S2. If the variation in amount of Hg in both formulations is taken into consideration, the increase of absorbance of Hg from S1 can be attributed to three time higher levels of Marich in it. The mean residual time (MRT) of blood Hg level is also higher in case of S1 [MRT= 81.5 min] than S2 sample [MRT= 70.03 min]. In the clinical study, S1 shows best results on the parameters of reduction in severity, breathlessness, cough, sputum, body position at night and labored breathing. S2 and S4 are moderately efficacious followed by S3. S2 and S4 are moderately useful in reduction of breathlessness and sputum, followed by S3. S1 and S2 are equally effective to improve in body position at night.
Conclusions : Marich is responsible for higher bioavailability of Hg from S1 sample. Process of triturating one by one Marich may also be responsible for higher level of Hg absorbance, from a comparatively less amount in the S1 formulation. Clinically the standard sample is effective on all parameters. This study has highlighted the basic principle of Pharmaceutics of Herbomineral complex drugs. The Rasashastriya processing method and proportions of ingredients peculiar to a formulation are designed to attain maximum efficacy. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2013 |
A Clinical study to evaluate memory enhancing effect of Brahmyadi Ghrita in healthy individuals. |
Dr. Sudhir Rasal |
Dr. Swati Gadgil, Assistant Professor, BVDUCOA |
NA |
Memory is the ability of an individual to record sensory stimuli; events, information etc. retain them over a short or long period of time and recall the same at later date when needed. Thus memory plays a very important role in one's life. Poor memory, lower attention and slow recall are common problems in today's stressful and competitive world. Medhya rasayana is a unique concept in Ayurved and Brahmyadi ghrita is one such formulation used as a medhya rasayana drug. In present study Brahmyadi ghrita was prepared, analysed and tested for its memory enhancement effect in healthy individuals of age group 40-50 years using pst Graduate Institute Memory Scale (M.D.I-MS) test. It was non randomized controlled clinical trials. Brahmyadi ghrita was administered in a dose of 10 gm twice a day with hot water for the duaration of 30 days. After analysing the data using paired T test and ANOVA followed by Tuckeys test brahmyadi ghrita demonstrated statistically significant memory enhancement effect when compared with plain control group (p<0.01) and cow ghee (p<0.05). Thus it can be concluded that brahmyadi ghrita can be used as a memory enhancer in healthy individuals and can be used as a medhya rasayana drug for prevention of probable memory loss due to aging. Key words: Medhya rasayana, M.D.I-MS, Brahmydi ghrita |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2006 |
Comparative Toxicological Evaluation of Shuddha Manahshila Processed in Bhringraj Swaras and Mixture of Bhringraj Swaras with Aja Mutra |
Dr. Deepali Suryawanshi |
Dr. Madhuri Dalvi-Pawar, associate professor |
NA |
Manashila (As2S2) is an Arsenic compound and is an ingredient of approximately 396 compound formulations. To formulate „shuddha form of Manashila in the present study swedan procedure was conducted using Bhringraj swaras and mixture of Bhringraj swaras and Aja mutra. Three samples were analyzed using physicochemical, SEM EDAX and XRD technique. Physicochemical properties of Ashuddha Manashila were matched with standards. Atomic % of different elements was observed in Ashuddha and both samples of Shuddha Manashila in SEM EDAX. XRD technique showed the structural change in Ashuddha and both samples of Shuddha Manashila. Toxicity profile of Ashuddha and two samples of Shuddha Manashila were determined using OECD guidelines 423 and 407 for Acute and Sub- acute toxicity respectively. In acute toxicity study Ladder system was followed. In the study LD50 of Ashuddha Manashila in rat was greater than 5000mg/kg rat. In sub-acute toxicity study limit test dose was considered and 1000mg/kg drug dose was administered to animals up to 28 days. Out of 8 parameters studied no significant abnormalities were observed in any of the 7 standard parameters viz. food consumption, average body weight, general observations, hematological investigation, percentage organ weight, gross necropsy and Histo-pathological investigations of all animals in all groups. In Biochemical parameter out of 18 values 4 values viz. Urea, Serum Creatinine, Serum Alkaline Phosphatage and Serum Triglycerides were altered but does not signify functional changes. Thus two samples of Shuddha Manashila did not show toxicity in Sub Acute toxicity Study. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2007 |
Development and Standardization of Shaman dhooma Varti- Devadarvyadi dhooma varti |
Dr. Gourav Gupte |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Deodarvyadi dhoomvarti is one of the orphic formulations unearth from Bhavprakash Nighantu. It is said to be effective clinically in chronic intense shwas. Its domain of action encompasses pranavaha strotas. Efficacy of Deodarvyadi dhooma is clinically proven though no strong evidence is available. Unavailability of definite standards makes it difficult to be used widely. Study regarding pharmaceutical standardization of Deodarvyadi dhoomvarti; consisting of powders of Cedrus deodara (heart wood), Sida cordifolia (whole plant), Nardostachys jatamansi (rhizomes) is carried out in this study. SOP was established for preparation of devdarvyadi dhoomvarti after several pilot studies. The standards were set with a view of present need, guided by ancient ayurvedic standards as length 9.5 cm, inner diameter 1.45 cm, outer diameter 1.95 cm and were converted from spindle shape to cylindrical.The smoke produced from combustion of Deodarvyadi varti was collected and was analyzed with the help of gas chromatography. Four peaks were observed. About 50 phytochemicals of the constituents of DDV are reported, the constituents actually delivered at the site of action is still unreported.
The study can conclude that Standard operating procedure was established for preparation of Deodarvyadi dhooma varti. Deodarvyadi dhoomvarti was modified and standardize. Even though the constituents of the smoke could not be confirmed, still the peaks observed by the study could be very surely used as one of the finger print studies for Deodarvyadi dhooma varti. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2018 |
Phramceutical, analytical study and exploratory clinical evaluation of Abha Guggulu prepared using Abha Phala in Janusandhigatavata |
Dr. Mrinmayee Hedaoo |
Dr. Trupti Patil |
Dr. Madhavi Mahajan , Dept Kayachikitsa, BVDUCOA |
Abha guggulu was prepared using pods of abha , by somapaka, suryapaka, agnipaka and compression. Analysis of all samples was carried out and interpreted. Administration of oral 3 g per day dose of abha guggulu, polyherbal combination of anti-arthritic and rejuvenating herbs proved to be significant in reduction of all parameters in WOMAC score in this exploratory single arm open label quasy experimental study in patients of osteoarthritis. AG did not exhibit any adverse drug reactions. Abha guggulu may be used as a safe herbal formulation for patients of osteoarthritis. This is the first research based on abha guggulu prepared using pods of abha. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2013 |
A Clinical Study of the effect of Vachadi Ghrita on Memory in Healthy Subjects. |
Dr Priyadarshini Magdum |
Dr. Madhuri Pawar, Associate Professor , BVDUCOA |
NA |
In traditional Ayurved, numbers of medicated ghee formulations are prescribed to regulate person’s cognitive functions. Vachadi ghrita [VG], a medicated ghee formulation is prescribed to maintain healthy process of intellect and memory. In recent experimental studies, positive action of VG on animal’s memory was proved. Thus to generate clinical evidence, the study was attempted to evaluate the therapeutic potential of VG on healthy individual’s memory using M.D.I memory scale test. Methods: Non randomized positive controlled clinical trial was carried out in ninety healthy individuals. Thirty participants from two groups received VG and Cow ghee respectively. Plain control group participants did not receive any drug. Drugs were administered orally in 10 g dose twice a day for continuous 30 days. M.D.I memory scale was used to analyze before and after treatment effects of drugs on learning and memory ability in healthy individuals. Results: The participants of Vachadi ghrita showed significant change in scores of M.D.I scale as compared to the participants of plain (p<0.01) and cow ghee groups (p<0.05). Substantial change in scores of mental balance, delayed – immediate recall, retention of similar and dissimilar pairs was determined in participants of VG group compared to other two groups. Study drug [VG] showed added positive effect on participant’s digestive power. Conclusion: Vachadi ghrita has significant effect on healthy individual’s learning ability, verbal working and short – term memory. The present results provide an evidence for therapeutic potential of VG on healthy person’s cognition. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2008 |
Evaluation of anti-histaminic and anti-inflammatory activity of standardized Talisadi Suspension without vanshalochan. |
Dr. Poonam Madan |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Talisadi Suspension without vanshalochan was prepared in our department and standardized. The present study aimed at assessment of its anti-inflammatory and anti-histaminic actions in Tamak Shwas (~Bronchial Asthma) using experimental animals. To study anti inflammatory activity, Granuloma pouch model in rats was used while to study anti histaminic activity protection against histamine induced bronchospasm model in guinea pigs was studied. It was evident that Talisadi Suspension possesses significant anti-inflammatory [P=0.0018] and anti-histaminic [P=0.0000109] activities. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2006 |
Experimental Toxicological evaluation of bhavana of Agasti Patra Swaras (Sesbania grandiflora) to Manahshila |
.Dr. Ekata Chauhan |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Manashila (As2S2) is an arsenic compound, which is an ingredient of approximately 396 formulations and is used in various dosage forms like taila, ghrita, lepa, avaleha, varti etc. Reports of toxicity of arsenic and arsenicals are available in plenty; which have led to restriction on usage of ‘Manashila’. However, no work has been reported on toxicological evaluation of Shuddha Manashila (Arsenic-bi-sulphide). In this study, toxicity profiles of Ashuddha and Shuddha Manashila were established and compared to evaluate role of bhavana of Agastipatraswarasa to detoxify Manshila. OECD guidelines number 423 and 407 for acute and sub acute toxicity were used for this study respectively. Acute Toxicity study revealed that Ashuddha Manashila is non toxic at 5000mg/kg dose. In the subacute study, no significant abnormalities observed in 7 parameters viz. average body weight per group, general observations, food consumption, hematological investigation, percentage organ weight, gross necropsy and histology. In Biochemical investigations out of 18 values, 15 values were found to be normal in all animals from all groups. Remaining 3 biochemical values viz. Urea, Serum Alkaline Phosphatage and Serum Triglycerides does not signify functional changes. Thus both the samples Shuddha and Ashuddha did not show toxicity in subacute toxicity study.
Role of ‘Bhavana of Agasti Patra Swaras’ to detoxify Manashila could not be established. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2021 |
Evaluation of Efficacy of Potentiated Brahmi Ghrita Using Animal Models |
Dr. Swati Gadgil |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Context: Siddha ghrita is the foremost drug of choice for preventive as well as curative
treatment for conditions involving cognition. However, the prime concern regarding siddha
ghrita is poor drug compliance due to its quite high dose, taste, smell, palatability, it can be
overcome by the process of potentiation which was tested in the present study.
Aim: To study the relationship between drug potentiation and reduction of dose or duration
or both with a case study of brahmi ghrita in chronic unpredictable mild stress (CUMS)
induced memory impairment and diazepam induced amnesia models.
Materials and Methods: Self prepared ekapaki (1x), panchapaki (5x) and dashapaki (10x)
brahmi ghrita (BG) were standardized using HPTLC analysis with bacoside A as a
biomarker. Standardized 1x BG (3.6 g/kg) and (5.0 g/kg) and 10x BG (3.6, 1.8 and 0.9 g/kg)
and (5.0, 2.5 and 1.25 g/kg) were evaluated in CUMS induced memory impairment in rats
and diazepam induced amnesia in mice respectively.
Results: HPTLC results displayed that concentration of bacoside A in the three formulations
was found increased as the number of paka increased (10x BG >5x BG>1x BG). One-way
ANOVA followed by Post hoc analysis was used for pre-clinical study. In CUMS model, 28
days exposure to CUMS significantly (P < 0.05) reduced the percentage of sucrose
consumption when compared with the non-stressed group. 10x BG at all 3 dose levels
showed significantly better results (P < 0.05) in comparison to 1x BG in memory test. In
diazepam model, 10x BG did not show significant results at half and one fourth of the
therapeutic dose in comparison with 1x BG on day nine. 10x BG (all three dose levels)
displayed significantly positive results (P < 0.01) compared to 1x BG on day seventeen.
Conclusion: Drug potentiation leads to increase in the concentration of biomarker in the final
formulation. 10x BG has displayed significant increase in efficacy in both the animal models.
This is the first study to report that drug potentiation increases the efficacy and achieves dose
reduction. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2010 |
Experimental Screening of Hingvadi Ghrita to Assess Antiepileptic and Nootropic Activity. |
Dr. Kavita S. Sawant |
Dr. Nandini More, Associate professor BVDUCOA |
NA |
Mental health is essential element of health according to ayurved as well as Modern science. Apsmar can be correlated with epilepsy as most of their signs &symptoms are same. Hingwadi ghrita (HG) is advocated for treatment of Apsamar. It contains Hingu, Sauvarchal lavan, Trikatu , Gomutra, & Goghruta. In the present study, an attempt was made to manufacture standard drug and analyze its anti-epileptic activity and effect on memory using animal models. HG was tested in 3dose level (X/2=0.05gm, X=0.1gm, 2X=0.2gm). For antiepileptic activitiy 2animal models, Increasing current Electroshock seizers(ICES) and PTZ induced convulsions in mice were used. In ICES model, HG significantly (p value <_ 0.0001) increased Seizure threshold current. In PTZ induced convulsions in mice model : HG at X/2 and X doses showed more protection than 2X dose: but the onset of convulsions was delayed in 2X dose. HG showed highly significant (p value <_0.0001) effect as compared to control drug in both models but less effective than standard drug. HG did not show memory impairment in Cook's pole climb avoidance in rat model as compared to Phenytion known antiepileptic activity. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2010 |
Evaluation of Antiepileptic and Nootropic Activity of Kushmand Ghrita using experimental models . |
Dr. Rupali S. Kale |
Dr. Swati Gadgil, Assistant Professor, BVDUCOA |
Dr. Kanchan Borule, |
Apasmar is a neuro -psychological disease, very well elaborated in Ayurved. Literal meaning of Apasmar is impairment of memory. Epilepsy can be corelated with it, as sign and symptoms are similar for both the disease. Modern antiepileptic drug supresses the seizure, but do not cure the disease and have some adverse effects, like cognitive impairment and sometimes need long life treatment. Thus, there is need for drugs which possess antiepileptic potential along with nootropic activity. Amalakyadi Ghrita (AG) containing Amalaki, Yashtimadhu and Goghrita has been recommended in the treatment of Apasmar. It is known to be efficacious in senile dementia.
Thus, the present study was aimed to manufacture standard drug (AG) and investigate its antiepileptic and nootropic activity. Antiepileptic activity was studied using two animal models. i.e Maximal Electric Shock (MES) and Pentylenetetrazole (PTZ) induced convulsion model in mice. Nootropic activity was studied using cooks pole climb avoidance in rat and Diazepam induced amnesia in mice model. It was evident that amalakyadi ghrita exhibited statistically significant antiepileptic activity when accessed using MES model (p ≤ 0.001). In PTZ model, AG at 2x dose level significantly delayed the onset of convulsion (p ≤ 0.05). In cooks pole climb avoidance in rat model AG at X dose level showed highly significant Nootropic activity (p ≤ 0.01). In Diazepam induced amnesia model, AG also exhibited statistically significant nootropic activity (p 0.001).
In conclusion amalakyadi ghrita exhibited dose dependant protection against electric and chemical induced seizures indicating its antiepileptic activity and proved as a potent nootropic drug. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2005 |
Evaluation of standardized syrup and suspension form of talisadi churna using experimental models |
Dr. Omkar Kulkarni |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Talisadi churna is one of the commonest yet hardly studied formulation for the efficacy profile and mode of action of the drugs. It is used in disorders of Pranavaha and Annavaha Srotasa. In this study, an effort was made to develop Standardized Syrup and Suspension form of Talisadi churna and study its activity against common respiratory and GIT pathogens in vitro as well as using human serum. SOP and stability studies of Talisadi Syrup and Suspension were done. The formulations appeared stable on shelf till 1 year with respect to organoleptic & physicochemical parameters, and microbial contamination. Alcohol extract of Talisadi churna showed good bactericidal activity against 4 micro organisms tested; whereas water and petroleum ether extract did not show any activity. The formulations when tested, in a dose of 2 gm single administration, using human serum of the healthy volunteers at different time intervals after ingestion of the drugs, it showed no statistically significant activity against tested micro organisms (E. coli & Staph. Aureus). Talisadi Syrup and Suspension are good modification in respect of manufacturing cost (only 10-20%> cost of TC), stability, palatability and acceptability by the patients. The efficacy profile of the formulations still need to be explored. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2012 |
Assessment of Nootropic activity of Panchagavya Ghrita in animal models. |
Dr. Ashutosh Pandey |
Dr. Madhuri Pawar, Associate Professor , BVDUCOA |
Dr. Priti Dhande, associate professor, BVDU, mediacl college Pharmacology dept |
Panchagavya ghrita [M.D.G] is one of the medicated ghee formulations, contains five cow products; cow ghee, cow milk, cow urine, cow dung juice and cow curd. M.D.G was prepared as per established SOP and compared with standard values. Nootropic activity of Panchagavya Ghrita (M.D.G) was studied using Diazepam induced amnesia in mice and Morris Water Maze test in rat model. M.D.G also reversed successfully the amnesia induced by Diazepam (1mg/kg, i.p.). M.D.G was administered in three dose levels of M.D.G as X/2 (2.5gm/kg), X (5gm/kg) and 2X (10gm/kg) in mice and X/2 (1.75gm/kg), X (3.5gm/kg) and 2X (7.0gm/kg) in rats. In EPM test significant effect of 5gm/kg dose of M.D.G was determined and Piracetam and M.D.G at 3.5gm/kg drugs have significant memory enhancement action in MWM test. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2020 |
Characterization of Gold bhasma and its Bioavailability with four different Anupana (Media) |
Dr. Trupti Patil |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Svarṇa bhasma [Gold Bhasma] is widely used Āyurvedika medicine in single and compound form. However, its characteristic investigations are very limited and bioavailability in humans is not studied. Present study of marketed Svarṇa bhasma was aimed at its characterization and bioavailability with single oral 30 mg dose plain and with four different anupāna. The bhasma was prepared by classical kukkuṭapuṭa method of 14 incinerations of Au-Hg-Lemon juice amalgamation with sulphur. It was characterized by Āyurvedika organoleptic, gravimetric and physicochemical methods and studied for bioavailability using NIST-NCL (USA) Joint assay protocol -PCC 9, Version 1.1. In this randomized bioavailability trial, selected 30 healthy human subjects were allocated into 5 groups of 3 males and 3 females in each. Svarṇa bhasma was administered in single oral dose of 30 mg to each group as plain, with honey (2.5 gm), marica-ghee combination (250 mg and 2.5 gm), glucose (250 mg), and lactose (250 mg) respectively. Blood gold levels at 0, 1, 3 and 5 hours were assessed by ICP- MS. The results of characterization revealed that Svarṇa bhasma contains polycrystalline gold nanoparticles of 5-20 nm. The study drug contains 95 percent of elemental gold (Au) in leafy agglomerates. Presence of Si, Ag, Al, Ca, Cu, Fe, K, Mg, Mn, Na, P, Sr, Ti, and Zn, and absence of Hg and S was also confirmed. Average Cmax was observed at 3 hours and Cmin at 5 hours. It is concluded that investigated Svarṇa bhasma contains highly crystalline spherical pure gold nanoparticles of 5-20 nm. The anupāna honey showed maximum Cmax (Average Cmax=0.002333 mcg/l) followed by plain dose (Cmax=0.000667 mcg/l), and marica-ghee (Cmax=0.000333). Nil to minimal absorption was observed in lactose and glucose groups. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2013 |
A Study of Medhya Activity of Vachadi Ghrita on Learning and Memory of High School Children. |
Dr. Santosh Pusadkar |
Dr. Nandini More, Associate professor BVDUCOA |
NA |
Depressed memory, lesser retention and slow recall are frequent problems in today’s stressful and competitive world. Children are also facing same problem in this competitive era in concentration on study. It’s necessary to add any substitute to enhance their memory and learning capacity. Brahmi is a famous plant used for memory enhancing property and increase in concentration. It is also used in combination with other herbs to increase its potency on CNS. Apart from regularly used formulation Brahmi Ghrita, a different one titled Brahmyadi Ghrita comprising of Brahmi, Siddharthak, Vacha, Sariva, Kushtha, Pippali, Saindhav lavan; processed in cow ghee is mentioned in Ashtanghridaya especially for various CNS conditions of children. Thus, the present study was undertaken to prepare & standardized the drug (BG) and investigates the effects on cognitive function of children.Improvement in learning and memory activity was studied using JPIP test for numerical ability and test of higher mental ability in science in children of age group 10 to 16 years. BG of study group and goghrita of control group was administered in one dose of 10 gms per day to participant. Scores was recorded and analyse statistcally by using‘t’ test and ANOVA for result. In this study it showed that learning and memory is significantly (p < 0.005) increased in children after 30 days of BG treatment of study group compared to goghrita of control group and plain group. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2008 |
Experimental evaluation of anti-histaminic and anti-inflammatory activity of Vasarishta. |
Dr. Swapnil Shinde |
Dr. Madhuri Dalvi-Pawar, associate professor |
NA |
Vasarishta’; a polyherbal fermented product prepared by addition of Vasa leaves [Adhatoda vasica] juice in Mritasanjivani Sura is prescribed to treat respiratory disorders especially Tamak Shwasa [Bronchial Asthma]. Previously therapeutic efficacy of Vasarishta in Tamak Shwasa in comparison with other dosage forms is clinically evaluated. However exact mechanism of pharmacological activity of the formulation is not evaluated till date. Hence in present study Vasarishta is assessed experimentally for anti-inflammatory and anti-histaminic activity using animal models. ‘Vasarishta’ was procured from market and analyzed in the laboratory. Sp. Gravity, pH, Total reducing sugar, Ethanol percentage and organoleptic tests were conducted and values are established. To study Anti-histaminic activity the model selected is Histamine induced Bronchial Hyper-reactivity in Guinea pigs. Animals are treated for 21 days with 0.0775ml/kg dose of Vasarishta. The anti-inflammatory activity is tested in cotton wool granuloma model. Vasarishta is administered for 7 days in the dose 0.09ml/kg. The obtained results are statistically analyzed by unpaired-‘t’ test. It is found that ‘Vasarishta’ has significant anti-inflammatory and highly significant anti-histaminic activity. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2017 |
Comparative assessment of two samples of Saraswatarishta on mental efficiency in elderly individuals |
Dr. Aishwarya Koyande |
Dr. Swati Gadgil, Assistant Professor, BVDUCOA |
NA |
Introduction- With an increase in the number of dependent elderlies facing enumerable health issues, Ayurveda can contribute a lot to improve their quality of life. With the prospect of worsening the situation of elderlies in the coming decades, ways and means of managing their health issues effectively needs to be examined and executed. The present study was aimed to prepare, analyse and compare the efficacy of two samples of Saraswatarishta on mental efficiency, memory function and insomnia in elderly individuals.
Material and Methodology: Saraswatarishta and SwarnaSaraswatarishta were prepared using the classical reference. Both were analysed using physico-chemical parameters and SwarnaSaraswatarishta with ICP-MS and the data was generated. Further efficacy of both the samples was evaluated clinically (10ml /day). Study was open labelled randomized clinical trial with 24 sample size of age group 55yrs and above. The three scales M.D.I-BAMEE, M.D.I-MS and RIS were used before and after the treatment of 30 days.
Results: The comparative efficacy of Saraswatarishta and SwarnaSaraswatarishta on mental efficiency, memory function and insomnia when assessed using three scales was found to be non-significant (p> 0.05). On intra group comparison, Saraswatarishta was found effective on memory function (p< 0.001) and Insomnia (p<0.01). Whereas, SwarnaSaraswatarishta was found effective on mental efficiency (p< 0.001), memory function (p< 0.001) and insomnia (p<0.01). SwarnaSaraswatarishta was also effective in improving appetite, bowel habits and reduction in time to fall asleep.
Conclusion: The SOP for Saraswatarishta was developed and validated through physicochemical analysis. Evidence of detection of Swarna in SwarnaSaraswatarishta using ICP-MS technique was established. In the present study, Saraswatarishta and SwarnaSaraswatarishta individually showed positive results in mental efficiency, memory and Insomnia after 30 days treatment but when compared were equi-effective. Being an exploratory study with just 24 sample size a firm conclusion regarding comparative efficacy cannot be made. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2005 |
Evaluation of pharmacological and anti microbial activity of standarzied Talisadi churna and Guti without vanchalochan |
Dr. Vidya Omna |
Dr. Madhuri Dalvi-Pawar, associate professor |
NA |
In the present study standardization of two dosage forms i.e Talisadi churna and Guti without Vanshalochan was carried out. Total Standardization study establishes analytical standards for Talisadi churna and Guti. In the Pilot study, Talisadi churna extracts were tested using Agar well diffusion method in four microorganisms [Staphylococcus, Pseudomonas, Kleibshella, Escherichia coli] to assess antimicrobial activity. It showed significant activity against Staphylococcus, Escherichia coli. Hence antimicrobial activity of Talisadi churna and Guti were tested experimentally using human serum against Staphylococcus, Escherichia coli . Zone of inhibition of Talisadi churna and Guti against both microorganisms was not statistically significant. Study concluded that this technique is not fruitful to assess this type of pharmacological activity of Talisadi churna and Guti. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2011 |
Experimental Evaluation of Nootropic Activity of Vachadi Ghrita and its In-vitro study using Biochemical Assay. |
Dr. Kunal Gurav |
Dr. Madhuri Dalvi-Pawar, associate professor |
Dr. Yogita Karandikar, Assistant professor, BVDU, Medical College |
Vachadi ghrita is medicated ghee ayurvedic formulation,recommended to improve cognition.Synergism of herbal drugs of preparation of VG and extraction of lipid soluble extractives of these drugs in Goghrita [Cow Ghee] may show cumulative positive effect on cognition. Thus the cognitive-enhancing activities of medicated ghee formulation VG was evaluated in Morris water maze test and in diazepam induced amnesia mice model using Elevated plus maze.Piracetam, most widely used nootropic agent as positive control and Goghrita as negative control was employed in both tests. VG was given in X/2, X and 2X dose levels.Study findings suggest that Vachadi Ghrita at therapeutic dose, exhibited anti-amnesic and memory enhancement activity in EPM and MWM tests. Hence it can be employed as nootropic drug or adjuvant to conventional treatment to minimize adverse effects of these drugs |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2010 |
Experimental Evaluation of Antiepileptic and Nootropic Activity of Panchagavya Ghrita. |
Dr. Amit R. Pawar |
Dr. Madhuri Dalvi-Pawar, associate professor |
Dr. Priti Dhande, Associate Professor, BVDU, Medical College |
Panchygavya Ghrita [M.D.G] is used in traditional Indian medicine to treat neurological disorders like epilepsy, schizophrenia, anxiety, depression, insomnia etc. M.D.G alone (in different dose) and as Polyherbal formulations has been studied for its antiepileptic activity but with variable results. As anti epileptics are known to cause memory impairment, the effect of on animal memory also needs to be studied. Methodology: Antiepileptic activity of M.D.G in 3 doses (2.5, 5 and 10 gm/kg) was estimated using ICES (Increasing current electro shock seizure) animal model wherein mice were treated with drugs for seven days. Animals were observed for occurrence of the THLE (tonic hind limb extension) at particular STC (seizure threshold current) in ICES test. M.D.G in 3 doses (1.8, 3.6 & 7.2 mg/kg) was also evaluated for its effect on memory after its administration for 21 days orally using Cook’s pole avoidance rat model. Results: M.D.G at 5 gm/kg dose showed comparable results to that of standard drug Phenytoin in ICES model by increasing the STC. Avoidance responses were significantly reduced by Phenytoin (p<0.05) after 21 days treatment as compared to all 3 doses of M.D.G. Conclusion: 7 days treatment with M.D.G in 5 gm/kg dose has shown anti epileptic activity in ICES animal model. M.D.G did not show deleterious effect on animal memory in Cook’s pole climbing apparatus on 21 days of treatment as compared to Phenytoin |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2009 |
Experimental Evaluation of Unmadgajakesari Rasa [UGK II] A Herbo-Mineral compound for its Antiepileptic and Effect on Learning Memory skills. |
Dr.Amit Jain |
Dr. Nandini More, Associate professor BVDUCOA |
NA |
Present study was done to evaluate Antiepileptic activity & Learning memory effect of Unmadgajakesarirasa II (Rasakamdhenu unmadchikitsa 9-12). Cook’s & Widely model was used to evaluate learning memory activity. Total 30 wistar rats were classified into 5 groups, control I & II, standard, test x & 2x, each containing 6 rats. Human dose of UGK-II was extrapolated using factor 0.018. After dosing; learning & relearning trials were given to all groups. The avoidance, escape and no response were observed and compared. Anti-epileptic activity was tested using electro shock model (ESM) and pentylenetetrazole induced convulsion (PC) model. For each study, 24 albino mice were classified into 4 groups having 6 mice each, into control, standard drug, test drug x dose and 2x dose. for both studies the human dose was extrapolated using factor 0.0026. For ESM study, the duration of tonic hind limb extension (THLE) & mortality was observed for 15min.The complete inhibition of THLE was considered as positive criteria. For the PC model, the time required for clonic convulsion, incidence & mortality was observed for duration of 30 mins. It has been concluded that UGK II has effective role in learning & memory activity. At both dose levels it proves equieffective with standard drug Phenytoin to abolish THLE. Also, when compared to control group and sodium valproate, both dose levels of UGK II have shown to reduce onset of time of convulsion. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2006 |
Experimental toxicological evaluation of Shuddha Manahshila processed sequentially in Bhringaraj, Jayanti ,Agasti and Aadrak swaras |
Dr. Yogendra Kasat |
Dr. Nandini More, Associate professor BVDUCOA |
NA |
Manashila (Realgar) is one of the mineral drugs which are used in the treatment of various diseases. As per modern science Arsenic is a toxic substance when it is in compound form. It is documented that absorbed Arsenic is accumulated in liver, kidneys, heart, lungs and deposited in gastrointestinal tract, spleen, lungs, and keratin rich tissues. Hence there is a need to determine toxicological profile of Manashila.In Ayurved, Shodhan Sanskar [detoxification process] is done on Manashila before using as a medicine to remove toxic effects. Present study focuses on acute toxicity study of Shuddha Manashila. Raw drugs were identified, selected, procured and authenticated by Ayurvedic and modern parameters. Detoxification of Manashila was done by boiling it in Bhringaraj, Agasti, Jayanti and Ardraka swaras sequentially, each for 12 hours in Dolayantra. Change in Colour, weight loss and analysis of Manashila indicate changes in properties of Manashila during Swedan Sanskar. Acute toxicity study was completed following OECD guidelines, with prior permission of Animal Ethics Committee. Effect of Manashila on body weight of rats, behaviour ,food consumption was not significance. Haematology and blood chemistry KFT, LFT, BSL, Lipid profile were done on 29 day. Values of blood urea, serum creatanin do not change. SEM EDAX, XRD reports and results of toxicity study revealed that LD50 dose of Manashila is > 5000mg/kg of rat. Manashila is non-toxic for therapeutic dose. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2014 |
Clinical Pharmocological Assessment of Hingwadi Ghrita in Mild to Moderate Depression. |
Dr. Roshni Takale |
Dr. Asmita Wele , Professor, BVDUCOA |
NA |
Depression is a disorder of major public health importance, in terms of its prevalence and the suffering, dysfunction, morbidity, and economic burden. There is no single cause of depression. Brain chemistry, hormones, and genetics may all play a role. Other risk factors for depression low self-esteem, anxiety disorder, borderline personality disorder, post-traumatic stress disorder (PTSD), physical or sexual abuse, chronic diseases like diabetes, multiple sclerosis, or cancer, alcohol or drug abuse, certain prescription medications, family history of depression include. Ayurveda describes two mental disorders Unmada and Apasmar.Depression correlates with premonitory symptoms of Unmada and Vishada. Study was aim to evaluate Hingwadi Ghrita in Mild to Moderate Depression.For assessment Hamilton Scale Test was used. This test was used to diagnose the severity of depression in patients. The contents of HG formulation are predominant in Katu Rasa and Ushna Virya. Review of researcheson the ingredients of HG show that these drugs individually seem potent enough, to control varied dysfunctions of the mind. HG was prepared following SOP and analytical values of HG were complied with the standard values. HAM-D test was taken before, mid & after completion of ghrita consumption. Patients of mild to moderate depression were non- randomly divided into two groups 6 patients in each group, group A- study group- HingwadiGhrita + standard antidepressant drug and group BStandard Antidepressant Drug. In group A patients HingwadiGhrita was administered 10 gm bid with lukewarm water for 60 days and for Koshthashuddhi purpose 1gm of Gandharva Haritaki churna with lukewarm water was given for five days at bedtimebefore administration ofHG. This study shows ‘decrease in score’ of Hamilton Scale Test Hingwadi Ghrita at dose 10 gm bid for 60 days with standard antidepressant drug has shown significant (P=0.0001) antidepressant activity in patients of mild to moderate depression. Secondary outcome of the study has shown ‘changes in certain secondary parameters’, which were assessed on the basis of ‘SamyakSnigdha Lakshana’. Easy evacuation of bowel and normal appetite were seen. |
NA |
| Bharati Vidyapeeth deemed to be University, college of Ayurveda, Pune |
Pune |
Maharashtra |
M.D. |
2004 |
Standardization of Shwaskuthar Rasa prepared with different concentrations of contents especially of Marich (Piper nigrum, Lin) |
Dr. Sheeba Varghese |
Dr. Madhuri Dalvi-Pawar, associate professor |
NA |
Shwaskuthara Rasa is a herbomineral formulation reported in Ayurvedic texts as well as in Ayurvedic formulary of India, part 1, 20:49. It contains Maricha [Piper nigrum] as a main ingredient, along with detoxified Parada [Mercury], detoxified Gandhaka [Sulphur], detoxified Vatsanabha [Aconitum ferox], purified Tankan [Borax], detoxified Manashila (Realgar), Shunthi (Zingiber officinale) and Pippali (Piper longum). It is advocated to treat respiratory disorders especially for Tamak Shwas [Bronchial Asthma]. Variant in the ratio of the contents of the formulation is stated in literature. In present study three samples [S1,S2,S3] of Shwaskuthar rasa were prepared with different proportions of the ingredients mainly of Marich [Piper nigrum] and fourth sample was procured from market. Routine clinical use of formulation has been observed but the standard quality parameters for this medicine are not in existence. Therefore an attempt was made to develop a suitable analytical method for the qualitative and quantitative estimation of active constituents of SKR. High performance thin layer chromatography, SEMEDAS, XRD techniques were used to standardize the formulation. Percentage of Piperine, Shagoal and Arsenic in four samples of SKR were established and compared. It is proved that sample prepared by dry trituration with addition of Marich seed one by one to other ingredients showed significant quantitative increase in the Piperine and Shaogal content in comparison with other three samples. |
NA |
